Fig. 2

Overview of reduced immunogenicity in iCD8 T cells through gene editing. To circumvent immune rejection, genetic editing is employed to knock out HLA class I and HLA class II molecules, rendering iCD8αβ + T cells unrecognizable by T cells through TCR-mediated recognition. Immune responses from NK cells are addressed by introducing the HLA-E gene and knocking out NK cell activation ligands expressed on the surface of iCD8αβ + T cells (triple KO). These gene editing strategies are effective at inhibiting the direct recognition of host immune cells and the mobilization of immune cells via CD4 + T cells, thus suppressing cytotoxic activity and preventing the elimination of tKO/E-iCD8αβ + T cells